Polymerase Error Rate

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10-4for Klenow fragment, and 2.1 x 10-4for Taq polymerases after a 106-fold amplification. The error rate for T4 DNA polymerase was not more than3 x 10-6.

Jan 6, 2017. For example, the reported error rate for Taq DNA polymerase I, can range over 10-fold, from 1 × 10−5 to 2 × 10−4 errors/base/doubling [9, 13].

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Why are there are so many genotypes and viruses? Some viruses purposefully use an error-prone polymerase (enzyme used to replicate DNA/RNA) to accelerate their mutation rate. For example, influenza pandemics occur when a “genetic.

. the poliovirus 3D polymerase. The high error rates of 3D polymerase result in high sequence diversity such that virus populations exist as quasispecies. This phenomenon is thought to facilitate survival of the virus population in complex.

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Selection of reliable reference genes for quantitative real-time polymerase chain reaction studies in maize grains

The data report directly on polymerase dynamics. showing a median accuracy of 99.3%, with no systematic error beyond fluorophore-dependent error rates.

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DNA – “There’s a trade-off between the error rates, how much correction you need. because they often produce secondary structures that interfere with synthesis.

Polymerase Fidelity: What is it, When the polymerase recognizes an error, The error rate per base incorporated was determined after calculating the.

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The table above demonstrates the low error rate of Phusion DNA Polymerase. After 30 cycles of PCR amplifying a 3 kb template, only 3.96 % of the product DNA molecules.

High-fidelity PCR utilizes DNA polymerases that couple low misincorporation rates. The error rate per base incorporated was determined after calculating the.

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Results. The majority of patients are 15 to 44 years of age (49.9% male), and we estimate that the case fatality rate is 70.8% (95% confidence interval [CI], 69 to 73.

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